PseKRAAC provides easier application for inputting sequences by accepting protein sequences in FASTA format via directly enter into the input text box or upload it as a FASTA file. The server also is capable of outputting result files in LIBSVM, CSV format or reduced protein sequences(FASTA format) for further analysis. For the convenience of the vast majority of experimental scientists, we also provided a step-by-step guide on how to generate the desired Pseudo K-tuple Reduced Amino Acid Composition(PseKRAAC).

Step 1:
    Open the web server at, and you will see the top page of PseKRAAC on your computer screen, as shown in following printscreen.

Step 2:
    Select the desired value for each parameter of PseKRAAC by using the drop-down menu: K-tuple, g-gap, and λ-correlation. The value must be a non-negative integer and smaller than L, where L is the length of the protein sequence concerned. Then select the types of reduced amino acid alphabet and the cluster size by clicking on the value/values in the chart.

Step 3:
    Enter your query protein sequences either into the input box or upload them as a file via the ‘‘Upload’’ button. The format should be in FASTA format.

Step 4:
    Once the parameters are selected and the query protein sequences are uploaded, click on the Submit button to see the results. If selecting type 1 reduced amino acid alphabet with 10 cluster groups(RAAC=10) and dipeptide (K=2), you will get 102= 100 discrete numbers for each of the protein sequences inputted.

Step 5:
    This server provide three types of outputting files, LIBSVM, CSV and FASTA format, users can select their desired result easily.